Development and Application of Expressed Protein Ligation

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holford4Minakhin L, Camarero JA, Holford M, Parker C, Muir TW, Severinov K. Mapping the Molecular Interface between the Sigma (70) Subunit of E. coli RNA Polymerase and T4 AsiA. J Mol Bi 2001 MAR 2:304(4):631-642.

holford7Huse M, Holford MN, Kuriyan J, Muir TW. Semisynthesis of Hyperphosphorylated Type I TGF Beta Receptor: Addressing the Mechanism of Kinase Activation. J Am. Chem. Soc 2000 AUG 30:122(34):8337-8338.

pic4Ayers B, Blaschke UK, Camarero JA, Cotton GJ, Holford M, Muir TW. Introduction of Unnatural Amino Acids into Proteins Using Expressed Protein Ligation. Biopolymers 1999:51(5):343-354.

holford5Holford MN, Muir TW. Adding ‘Splice’ to Protein Engineering. Structure AUG:6(8):951-956

Holford Laboratory at CUNY Hunter College